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KMID : 1123920030170010213
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Korean Journal of Oriental Physiology and Pathology
2003 Volume.17 No. 1 p.213 ~ p.219
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Induction of Cdk inhibitor p27 and Inhibition of pRB Phosphorylation by Insamsapye-tang Treatment in Human Lung Cancer A549 Cells
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Lee Min-Woo
Seo Chang-Hun
Park Cheol
Lee Won-Ho
Choi Yung-Hyun
Park Dong-Il
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Abstract
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We investigated the effects of Insamsapye-tang (ISSPT) water extract on the cell proliferation of human lung carcinoma A549 cells. ISSPT treatment resulted in the inhibition of cell proliferation in a concentration-dependent manner. This anti-proliferative effect of A549 cells by ISSSPT treatment was associated with morphological changes such as membrane shrinking and cell rounding up. DNA flow cytometric histograms showed that population of G1 phase of the cell cycle was increased by ISSPT treatment in a concentration-dependent manner. ISSPT treatment induced the levels of tumor suppressor p53 protein and cyclin-dependent kinase (Cdk) inhibitor p27 without significant alteration of cyclins and Cdks expression. In addition, ISSPT treatment resulted in down-regulation of phosphorylated retinoblastoma protein (pRB). However, the levels of p130, the pRB family protein, and transcription factors. E2F-1 and E2F-4. were remained unchanged. The present results indicated that ISSPT-induced inhibition of lung cancer cell proliferation is associated with the blockage of G1/S progression and the induction of apoptosis, and we suggest that ISSPT will be an effective therapeutic agent on human lung cancer.
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KEYWORD
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Insamsapye-tang(人蔘瀉肺湯), cell cycle, p27, pRB, human lung cancer
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